| 02:45 | Worksop Town – Bedford Town | 1:2 |
| 02:45 | Spennymoor Town – Spalding United | 1:3 |
| 02:45 | Southport – Morecambe | 2:1 |
| 02:45 | South Shields – Marine | 2:1 |
| 02:45 | Radcliffe Borough – Hebburn Town | 5:1 |
| 02:45 | Macclesfield – Scarborough Athletic | 0:0 |
| 02:45 | Weston super Mare – AFC Totton | 3:1 |
| 02:45 | Tonbridge Angels – Maidstone United | 0:1 |
| 02:45 | Salisbury City – Slough | 5:1 |
| 02:45 | Maidenhead – Torquay | 0:4 |
| 02:45 | Hampton & Richmond – Farnborough FC | 2:0 |
| 02:45 | Folkestone Invicta – Horsham | 1:1 |
| 02:45 | Ebbsfleet United – Hemel Hempstead | 0:0 |
| 02:45 | Dorking Wanderers – Chelmsford City | 3:3 |
| 02:45 | Dagenham and Redbridge – Dover Athletic | 0:1 |
| 02:45 | Chesham United – Billericay | 1:2 |
| 02:45 | King's Lynn – Hednesford Town | 2:3 |
| 02:45 | Harborough Town – Hereford | 0:3 |
| 02:45 | Chorley FC – Darlington | 5:2 |
| 02:45 | Chester – Merthyr Town | 0:1 |
| 02:45 | Buxton FC – Brackley Town | 5:0 |
| 02:45 | AFC Telford – Oxford City | 1:0 |