| 02:45 | Biggleswade FC – Three Bridges | 1:3 |
| 03:00 | Aveley – Ware | 3:1 |
| 22:00 | Hanwell Town – Enfield Town | 2:4 |
| 22:00 | Poole Town – Basingstoke | 1:0 |
| 22:00 | Newmarket Town – Leighton Town | 2:1 |
| 22:00 | Waltham Abbey – Afc Croydon Athletic | 7:1 |
| 22:00 | Real Bedford – Wingate & Finchley | 0:3 |
| 22:00 | Takeley – Hitchin Town | 2:0 |
| 22:00 | Havant & waterloovile – Taunton Town | 2:3 |
| 22:00 | Eastbourne Borough – Welwyn Garden City | 4:2 |
| 22:00 | Maldon & Tiptree – Egham Town | 0:2 |
| 22:00 | Moneyfields – Wimborne Town | 1:3 |
| 22:00 | Berkhamsted – Chatham Town | 3:1 |
| 22:00 | Plymouth Parkway – Bath City | 0:3 |
| 22:00 | Leiston – Dartford | 0:3 |
| 22:00 | Hastings Utd – Dulwich Hamlet | 2:1 |
| 22:00 | Canvey Island – Needham Market | 0:3 |
| 22:00 | St Albans City – Bury | 4:1 |
| 22:00 | Halesowen Town – Wellingborough Town | 2:0 |
| 22:00 | Alfreton – Shifnal Town | 2:1 |
| 22:00 | Hanworth Villa – Bishop's Stortford | 3:2 |
| 22:00 | Gloucester – Stafford Rangers | 4:0 |
| 22:00 | Burgess Hill Town – Winslow United | 6:2 |
| 22:00 | Whyteleafe – Whitehawk | 1:4 |
| 22:00 | Racing Club Warwick – Bromsgrove Sporting | 0:1 |
| 22:00 | Uxbridge – Lewes | 2:1 |
| 22:00 | Peterborough Sports – Banbury United | 1:3 |
| 22:00 | Quorn – Leamington | 4:2 |
| 22:00 | Ilkeston Town – Anstey Nomads | 1:3 |
| 22:00 | Stanway Rovers – Faversham Town | 0:2 |
| 22:00 | Tiverton Town – Gosport Borough | 1:3 |
| 22:00 | Thame United – Chichester City | 1:2 |
| 22:00 | Evesham Utd – Stratford Town | 3:1 |
| 22:00 | Matlock Town – Kettering | 0:4 |
| 22:00 | Worcester – Leek Town | 0:2 |
| 22:00 | Stamford AFC – Loughborough University | 1:2 |
| 22:00 | Bedworth United – Redditch Utd | 0:1 |
| 22:00 | Rushall Olympic – Stourbridge | 1:3 |
| 22:00 | Alvechurch – Bourne Town | 1:2 |
| 22:00 | Curzon Ashton – Stalybridge | 3:1 |
| 22:00 | Bootle – Afc Emley | 5:0 |
| 22:00 | Bridlington Town – Whitby Town | 3:0 |
| 22:00 | Warrington Rylands – Stockton Town | 3:1 |
| 22:00 | Guiseley AFC – Bamber Bridge | 4:2 |
| 22:00 | Cleethorpes Town – Lancaster City | 5:1 |
| 22:00 | Dunston Uts – Avro | 0:2 |
| 22:00 | Guisborough Town – Redcar Athletic | 0:1 |
| 22:00 | Workington – Blyth Spartans | 4:2 |
| 22:00 | Hyde – FC United | 2:0 |
| 22:00 | St Albans City – Bury Town | 4:1 |